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Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24479

Novus Biologicals, part of Bio-Techne
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NBP3-24479

Key Product Details

Sample Type & Volume Required Per Well

Animal tissue

Sensitivity

1.21 U/g fresh weight

Assay Range

1.21 - 40 U/g fresh weight

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (324 nm)
Assay time: 65 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.1

Inter-Assay Precision (Precision between assays) %CV < 9.4

Scientific Data Images for Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric) [NBP3-24479] -

Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric) [NBP3-24479] - Assay Example

Kit Contents for Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Inhibitor
  • Reagent 3: Substrate A
  • Reagent 4: Substrate B
  • Reagent 5: Accelerant A
  • Reagent 6: Accelerant B
  • Reagent 7: Chromogenic Agent
  • UV Microplate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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