GPT Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24458

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, animal tissue, cell samples and cell culture supernatant

Sensitivity

0.75 IU/L

Assay Range

0.75 - 72.3 IU/L

View all GPT Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (500 - 520 nm)
Assay time: 80 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.3

Inter-Assay Precision (Precision between assays) %CV < 9

Scientific Data Images for GPT Activity Assay Kit (Colorimetric)

GPT Activity Assay Kit (Colorimetric)

GPT Activity Assay Kit (Colorimetric) [NBP3-24458] -

GPT Activity Assay Kit (Colorimetric) [NBP3-24458] - Standard Reference Curve
GPT Activity Assay Kit (Colorimetric)

GPT Activity Assay Kit (Colorimetric) [NBP3-24458] -

GPT Activity Assay Kit (Colorimetric) [NBP3-24458] - Assay Example

Kit Contents for GPT Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: 2 mmol/L Sodium Pyruvate
  • Reagent 3: Substrate Solution
  • Reagent 4: Chromogenic Agent
  • Reagent 5: Alkali Readent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GPT

GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.

Alternate Names

AAT1alanine aminotransferase 1, ALT1, ALT1EC 2.6.1.2, Glutamate pyruvate transaminase 1, glutamic-alanine transaminase 1, Glutamic--alanine transaminase 1, glutamic-pyruvate transaminase (alanine aminotransferase), Glutamic--pyruvic transaminase 1, GPT1GPT 1

Gene Symbol

GPT

Additional GPT Products

Product Documents for GPT Activity Assay Kit (Colorimetric)

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Certificate of Analysis

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Product Specific Notices for GPT Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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