PLA2G2A Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1307

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Tissue Homogenates, Cell Lysates (10 uL/well. All samples should be assayed in duplicate at minimum.)

Assay Range

3.5 to 42 nmol/min/ml

View all PLA2G2A Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Assay Kit (Colorimetric)

Reactivity

Mouse

Specificity

Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone unless specific inhibitors or purification procedures are used.

Description

Phospholipases A2 (calcium-dependent cytosolic) Assay Kit is a colorimetric method for assaying the activity of cPLA2.

Kit Contents for PLA2G2A Assay Kit (Colorimetric)

  • 96-Well Cover Sheet
  • 96-Well Solid Plate (Colorimetric Assay)
  • Arachidonoyl Thio-PC (Substrate)
  • Bee venom PLA2 Control
  • Bromoenol Lactone Solution
  • cPLA2 Assay Buffer
  • DTNB/EGTA

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PLA2G2A

Phospholipases A2 (PLA2s) catalyze the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids, yielding a free fatty acid and a lysophospholipid as products. The release of arachidonic acid from membrane phospholipids by these enzymes is believed to be the key step in the biosynthesis of eicosanoids. There are primarily three different kinds of PLA2s, they are secretory (sPLA2), calcium-dependent cytosolic (cPLA2), and calcium-independent (iPLA2) PLA2s. Of these three different types of enzymes, only the cPLA2 exhibits specificity towards arachidonic acid whereas all others can hydrolyze any fatty acid at the sn-2 position.

Long Name

Phospholipase A2 Group IIA

Alternate Names

Mom1, PLA2B, PLA2L, RASF-A

Gene Symbol

PLA2G2A

Additional PLA2G2A Products

Product Documents for PLA2G2A Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLA2G2A Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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