11 beta-HSD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-934

Novus Biologicals, part of Bio-Techne
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NB300-934
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 11 beta-HSD1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CTSYNMDRFINK corresponding to C-Terminus according to NP_005516.1, NP_861420.1.

Specificity

This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 11 beta-HSD1 Antibody

Western Blot: 11 beta-HSD1 Antibody [NB300-934]

Western Blot: 11 beta-HSD1 Antibody [NB300-934]

Western Blot: 11 Beta HSD1 Antibody [NB300-934] - (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for 11 beta-HSD1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 32.4 kDa band according to NP_005516.1 and NP_861420.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 11 beta-HSD1

The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Long Name

11 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

11 betaHSD1, HSD11B1

Entrez Gene IDs

3290 (Human)

Gene Symbol

HSD11B1

UniProt

NP_005516 (Human)

Additional 11 beta-HSD1 Products

Product Documents for 11 beta-HSD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 11 beta-HSD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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