12-Lipoxygenase Antibody (2D10)
Novus Biologicals, part of Bio-Techne | Catalog # H00000239-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2D10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ALOX12 (NP_000688, 564 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
Specificity
ALOX12 - arachidonate 12-lipoxygenase (2D10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for 12-Lipoxygenase Antibody (2D10)
ELISA: 12-Lipoxygenase Antibody (2D10) [H00000239-M01] - Detection limit for recombinant GST tagged ALOX12 is approximately 0.3ng/ml as a capture antibody.
Applications for 12-Lipoxygenase Antibody (2D10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 12-Lipoxygenase
Long Name
Polyunsaturated fatty acid lipoxygenase ALOX12
Alternate Names
12LO, 12S-lipoxygenase, 12S-LOX, ALOX12, EC 1.13.11.-, EC 1.13.11.31, EC 1.13.11.33, EC 3.3.2.-, LOG12
Entrez Gene IDs
239 (Human)
Gene Symbol
ALOX12
UniProt
Additional 12-Lipoxygenase Products
Product Documents for 12-Lipoxygenase Antibody (2D10)
Product Specific Notices for 12-Lipoxygenase Antibody (2D10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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