12-Lipoxygenase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35458
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 483-663 of human 12-Lipoxygenase (NP_000688.2).
Sequence:
YQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
Sequence:
YQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 12-Lipoxygenase Antibody
Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [NBP3-35458] -
Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [NBP3-35458] - Immunofluorescence analysis of paraffin-embedded rat lung using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] -
Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] -
Immunohistochemistry: 12-Lipoxygenase Antibody [NBP3-35458] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using 12-Lipoxygenase Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.Applications for 12-Lipoxygenase Antibody
Application
Recommended Usage
Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 12-Lipoxygenase
Long Name
Polyunsaturated fatty acid lipoxygenase ALOX12
Alternate Names
12LO, 12S-lipoxygenase, 12S-LOX, ALOX12, EC 1.13.11.-, EC 1.13.11.31, EC 1.13.11.33, EC 3.3.2.-, LOG12
Gene Symbol
ALOX12
Additional 12-Lipoxygenase Products
Product Specific Notices for 12-Lipoxygenase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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