14-3-3 beta/alpha Antibody (6F6)

Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] -

Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue.
All lanes: 14-3-3 beta/alpha Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 29, 28 kDa
Observed band size: 25-35 kDa

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] -

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] -

Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.