14-3-3 beta/alpha Antibody (SD0837)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67833

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67833
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD0837

Concentration

1 mg/ml

View all 14-3-3 beta/alpha Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to C terminal Human 14-3-3 beta/alpha aa 197-246 / 246. (SwissProt: P31946 Human; SwissProt: Q9CQV8 Mouse; SwissProt: P35213 Rat)

Localization

Cytoplasm, Melanosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 14-3-3 beta/alpha Antibody (SD0837)

Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Immunocytochemistry/ Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833]

Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
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Applications for 14-3-3 beta/alpha Antibody (SD0837)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 beta/alpha

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol.

Alternate Names

14-3-3 alpha, 14-3-3 beta, 14-3-3 protein beta/alpha, brain protein 14-3-3, beta isoform, GW128, HS1, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, protein kinase C inhibitor protein-1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide, YWHAA

Gene Symbol

YWHAB

Additional 14-3-3 beta/alpha Products

Product Documents for 14-3-3 beta/alpha Antibody (SD0837)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 14-3-3 beta/alpha Antibody (SD0837)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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