14-3-3 beta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99635
![Western Blot: 14-3-3 beta Antibody [NBP2-99635]](https://resources.bio-techne.com/images/products/14-3-3-beta-Antibody-Western-Blot-NBP2-99635-img0003.jpg "Western Blot: 14-3-3 beta Antibody [NBP2-99635]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 14-3-3 beta Antibody
Immunogen
Produced in rabbits immunized with purified, recombinant Human 14-3-3 beta (Accession#: NP_003395.1; Met1-Asn246).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for 14-3-3 beta Antibody
Western Blot: 14-3-3 beta Antibody [NBP2-99635]
Western Blot: 14-3-3 beta Antibody [NBP2-99635] - Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: 14-3-3 beta Antibody [NBP2-99635]](https://resources.bio-techne.com/images/products/14-3-3-beta-Antibody-Immunoprecipitation-NBP2-99635-img0004.jpg "Immunoprecipitation: 14-3-3 beta Antibody [NBP2-99635]")
Immunoprecipitation: 14-3-3 beta Antibody [NBP2-99635]
Immunoprecipitation: 14-3-3 beta Antibody [NBP2-99635] - 14-3-3 beta was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-14-3-3 beta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 beta rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDaApplications for 14-3-3 beta Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 beta
Alternate Names
1433 beta, GW128, HS1, YWHAB
Gene Symbol
YWHAB
Additional 14-3-3 beta Products
Product Documents for 14-3-3 beta Antibody
Product Specific Notices for 14-3-3 beta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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