14-3-3 eta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99642

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 eta Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human 14-3-3 eta (Uniprot#: Q04917; Gly2-Asn246).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 14-3-3 eta Antibody

Western Blot: 14-3-3 eta Antibody [NBP2-99642]

Western Blot: 14-3-3 eta Antibody [NBP2-99642]

Western Blot: 14-3-3 eta Antibody [NBP2-99642] - Anti-14-3-3 eta rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 31 kDa
Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99642]

Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99642]

Immunoprecipitation: 14-3-3 eta Antibody [NBP2-99642] - 14-3-3 eta was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg k562 Whole Cell Lysate. Lane D:0.5 mg NIH-3T3 Whole Cell Lysate. 2 ul anti-14-3-3 eta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 eta rabbit polyclonal antibody, at 1:2000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa

Applications for 14-3-3 eta Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 eta

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternate Names

1433 eta, Protein AS1, YWHA1, YWHAH

Gene Symbol

YWHAH

Additional 14-3-3 eta Products

Product Documents for 14-3-3 eta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 14-3-3 eta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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