14-3-3 tau/theta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90337

Novus Biologicals, part of Bio-Techne
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NBP1-90337
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 theta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 14-3-3 tau/theta Antibody

Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337]

Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337]

Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-90337]

Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-90337]

Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-90337] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.
Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337]

Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337]

Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Applications for 14-3-3 tau/theta Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 theta

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternate Names

14-3-3 protein tau, 1433 theta, HS1 protein, YWHAQ

Gene Symbol

YWHAQ

Additional 14-3-3 theta Products

Product Documents for 14-3-3 tau/theta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 14-3-3 tau/theta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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