14-3-3 zeta/beta Antibody (3J3J9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16769
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3J3J9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 zeta/beta (P31946). MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 14-3-3 zeta/beta Antibody (3J3J9)
![Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]](https://resources.bio-techne.com/images/products/14-3-3-zeta-beta-Antibody-3J3J9-Western-Blot-NBP3-16769-img0002.jpg "Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]")
Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]
Western Blot: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769] - Western blot analysis of extracts of various cell lines, using 14-3-3 zeta/beta Rabbit mAb (NBP3-16769) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]](https://resources.bio-techne.com/images/products/14-3-3-zeta-beta-Antibody-3J3J9-Immunocytochemistry-Immunofluorescence-NBP3-16769-img0001.jpg "Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]")
Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769]
Immunocytochemistry/Immunofluorescence: 14-3-3 zeta/beta Antibody (3J3J9) [NBP3-16769] - Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 zeta/beta Rabbit mAb (NBP3-16769) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for 14-3-3 zeta/beta Antibody (3J3J9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 14-3-3 zeta/beta
Alternate Names
KCIP-1MGC126532,14-3-3-zeta, MGC111427, MGC138156, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide, zeta polypeptide
Gene Symbol
YWHAZ
Additional 14-3-3 zeta/beta Products
Product Documents for 14-3-3 zeta/beta Antibody (3J3J9)
Product Specific Notices for 14-3-3 zeta/beta Antibody (3J3J9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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