15-Lipoxygenase 1 Antibody (3D8)
Novus Biologicals, part of Bio-Techne | Catalog # H00000246-M04
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ALOX15 (AAH29032, 1 a.a. ~ 662 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGKRLWPARGKETELKVEVPEYLGPLLFVKLRKRHLLKDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGVLSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYRWGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFEVSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGANPVVLRRSAHLPARLVFPPGMEELQAQLEKELEGGTLFEADFSLLDGIKANVILCSQQHLAAPLVMLKLQPDGKLLPMVIQLQLPRTGSPPPPLFLPTDPPMAWLLAKCWVRSSDFQLHELQSHLLRGHLMAEVIVVATMRCLPSIHPIFKLIIPHLRYTLEINVRARTGLVSDMGIFDQIMSTGGGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLGVKSSFYPQDALRLWEIIYRYVEGIVSLHYKTDVAVKDDPELQTWCREITEIGLQGAQDRGFPVSLQARDQVCHFVTMCIFTCTGQHASVHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRRQPVMVAVGQHEEEYFSGPEPKAVLKKFREELAALDKEIEIRNAKLDMPYEYLRPSVVENSVAI
Specificity
arachidonate 15-lipoxygenase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for 15-Lipoxygenase 1 Antibody (3D8)
![Western Blot: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-3D8-Western-Blot-H00000246-M04-img0005.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]")
Western Blot: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]
Western Blot: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Analysis of ALOX15 expression in Hela S3 NE (Cat # L013V3).![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-3D8-Immunohistochemistry-Paraffin-H00000246-M04-img0004.jpg "Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]")
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04]
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Analysis of monoclonal antibody to ALOX15 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml
ELISA: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Detection limit for recombinant GST tagged ALOX15 is approximately 3ng/ml as a capture antibody.
Applications for 15-Lipoxygenase 1 Antibody (3D8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 15-Lipoxygenase 1
Long Name
Polyunsaturated fatty acid lipoxygenase ALOX15
Alternate Names
12-LOX, 12/15-lipoxygenase, 15-LOX, 15-LOX-1, ALOX15, EC 1.13.11.12, LOG15
Entrez Gene IDs
246 (Human)
Gene Symbol
ALOX15
OMIM
152392 (Human)
UniProt
Additional 15-Lipoxygenase 1 Products
Product Documents for 15-Lipoxygenase 1 Antibody (3D8)
Product Specific Notices for 15-Lipoxygenase 1 Antibody (3D8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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