Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3G8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all 15-Lipoxygenase 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]

Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]

Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells
Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.
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Applications for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 15-Lipoxygenase 1

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as wellas on linoleic acid

Long Name

Polyunsaturated fatty acid lipoxygenase ALOX15

Alternate Names

12-LOX, 12/15-lipoxygenase, 15-LOX, 15-LOX-1, ALOX15, EC 1.13.11.12, LOG15

Gene Symbol

ALOX15

Additional 15-Lipoxygenase 1 Products

Product Documents for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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