15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70169
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0001.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]")
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0004.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Canine, Monkey, Primate
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3G8
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free
Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]
Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70169-img0005.jpg "Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]
Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0011.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]
Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0002.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8)Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0006.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0007.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0008.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0009.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0010.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]](https://resources.bio-techne.com/images/products/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0012.jpg "Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169]")
Applications for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 15-Lipoxygenase 1
Long Name
Polyunsaturated fatty acid lipoxygenase ALOX15
Alternate Names
12-LOX, 12/15-lipoxygenase, 15-LOX, 15-LOX-1, ALOX15, EC 1.13.11.12, LOG15
Gene Symbol
ALOX15
Additional 15-Lipoxygenase 1 Products
Product Documents for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free
Product Specific Notices for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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