15-Lipoxygenase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06976

Novus Biologicals, part of Bio-Techne
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NBP1-06976
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 15-Lipoxygenase 1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HYKTDVAVKDDPE corresponding to internal region according to NP_001131.3.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 15-Lipoxygenase 1 Antibody

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-06976] - (2ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for 15-Lipoxygenase 1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2-0.5 ug/ml
Application Notes
Western blot: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle and Kidney.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 15-Lipoxygenase 1

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as wellas on linoleic acid

Long Name

Polyunsaturated fatty acid lipoxygenase ALOX15

Alternate Names

12-LOX, 12/15-lipoxygenase, 15-LOX, 15-LOX-1, ALOX15, EC 1.13.11.12, LOG15

Entrez Gene IDs

246 (Human)

Gene Symbol

ALOX15

UniProt

NP_001131.3 (Human)

Additional 15-Lipoxygenase 1 Products

Product Documents for 15-Lipoxygenase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 15-Lipoxygenase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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