15-Lipoxygenase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90336

Novus Biologicals, part of Bio-Techne
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NBP1-90336
NBP1-90336-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all 15-Lipoxygenase 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RTVGEDPQGLFQKHREEELEERRKLYRWGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFEVSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 15-Lipoxygenase 1 Antibody

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Western Blot: 15-Lipoxygenase 1 Antibody [NBP1-90336] - Analysis in control (vector only transfected HEK293T lysate) and ALOX15 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336]

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody [NBP1-90336] - Staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
View 3 more images

Applications for 15-Lipoxygenase 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 15-Lipoxygenase 1

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as wellas on linoleic acid

Long Name

Polyunsaturated fatty acid lipoxygenase ALOX15

Alternate Names

12-LOX, 12/15-lipoxygenase, 15-LOX, 15-LOX-1, ALOX15, EC 1.13.11.12, LOG15

Entrez Gene IDs

246 (Human)

Gene Symbol

ALOX15

UniProt

P16050 (Human)

Additional 15-Lipoxygenase 1 Products

Product Documents for 15-Lipoxygenase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 15-Lipoxygenase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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