15-Lipoxygenase 2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92668

Novus Biologicals, part of Bio-Techne
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NBP2-92668-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 15-Lipoxygenase 2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human 15-Lipoxygenase 2 (NP_001132.2). MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 15-Lipoxygenase 2 Antibody - Azide and BSA Free

Western Blot: 15-Lipoxygenase 2 AntibodyAzide and BSA Free [NBP2-92668]

Western Blot: 15-Lipoxygenase 2 AntibodyAzide and BSA Free [NBP2-92668]

Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of extracts of various cell lines, using ALOX15B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 2s.
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668]

Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668]

Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of U2OS cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668]

Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668]

Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of NIH/3T3 cells using ALOX15B Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for 15-Lipoxygenase 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 15-Lipoxygenase 2

15 Lipoxygenase 2 encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

15-lipoxygenase 2, 15-LOX-B, arachidonate 15-lipoxygenase 2, arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, arachidonate 15-lipoxygenase, second type, arachidonate 15-lipoxygenase, type B, arachidonate omega(6) lipoxygenase, EC 1.13.11, EC 1.13.11.33,15-LOX-215S-lipoxygenase

Gene Symbol

ALOX15B

Additional 15-Lipoxygenase 2 Products

Product Documents for 15-Lipoxygenase 2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 15-Lipoxygenase 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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