4-1BB Ligand/TNFSF9 Antibody (AT113-2) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11986
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (AT113-2) - Chimeric - Azide and BSA Free [NBP3-11986]](https://resources.bio-techne.com/images/products/4-1BB-Ligand-TNFSF9-Antibody-AT113-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11986-img0001.jpg "Immunocytochemistry/ Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (AT113-2) - Chimeric - Azide and BSA Free [NBP3-11986]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # AT113-2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising LOU rats with soluble recombinant 4-1BB Ligand/TNFSF9:Fc fusion protein.
Specificity
This antibody is specific for 4-1BB ligand (also known as CD137 ligand), which is a member of the TNF superfamily and is a type II membrane protein. 4-1BB ligand is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation of these cells. Both of the 4-1BB ligand and its receptor, TNFRSF9/4-1BB, are involved in the antigen presentation process and in the generation of cytotoxic T cells. In direct ELISAs, no cross-reactivity with recombinant human RELT is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (AT113-2) - Chimeric - Azide and BSA Free [NBP3-11986]
Immunocytochemistry/Immunofluorescence: 4-1BB Ligand/TNFSF9 Antibody (AT113-2) - Chimeric [NBP3-11986] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of AT113-2 (NBP3-11986) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11986, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: 4-1BB Ligand/TNFSF9
Alternate Names
41BB Ligand, CD137L, TNFSF9
Gene Symbol
TNFSF9
Additional 4-1BB Ligand/TNFSF9 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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