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Key Product Details

Species Reactivity

All Species

Applications

Validated:

Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # AB3/63.3

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

5-hmC conjugated to BSA

Specificity

Expected to detect 5-hydroxymethylcytosine in all species.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for 5-hmC Antibody (AB3/63.3) - BSA Free

Application
Recommended Usage

Dot Blot

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
Positive control(s): Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-hmC

Alternate Names

5'-hmC, 5-Hydroxymethylcytosine

Additional 5-hmC Products

Product Documents for 5-hmC Antibody (AB3/63.3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-hmC Antibody (AB3/63.3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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