5-HT2C Antibody (9H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26213
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9H8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human 5-HT2C [UniProt P28335]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 5-HT2C Antibody (9H8)
Immunohistochemistry: 5-HT2C Antibody (9H8) [NBP3-26213] -
Immunohistochemistry: 5-HT2C Antibody (9H8) [NBP3-26213] - Image of 5-HT2C Antibody (9H8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: 5-HT2C Antibody (9H8) [NBP3-26213] -
Western Blot: 5-HT2C Antibody (9H8) [NBP3-26213] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.All lanes: 5-HT2C Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 52, 29 kDa
Observed band size: 75 kDa
Applications for 5-HT2C Antibody (9H8)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: 5-HT2C
Long Name
5-Hydroxytryptamine Receptor 2C
Alternate Names
5-HTR2C, 5HT2C, HTR2C, Serotonin Receptor 2C
Gene Symbol
HTR2C
Additional 5-HT2C Products
Product Documents for 5-HT2C Antibody (9H8)
Product Specific Notices for 5-HT2C Antibody (9H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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