5-MethylCytosine Antibody (RM231)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61470
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Vertebrate
Applications
Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Methylated DNA Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM231
Concentration
1 mg/ml
Product Summary for 5-MethylCytosine Antibody (RM231)
Immunogen
BSA-conjugated 5-MethylCytosine
Specificity
This antibody reacts to 5-MethylCytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 5-MethylCytosine Antibody (RM231)
Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Immunocytochemical staining of HeLa cells using NBP2-61470 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Dot Blot: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
Dot Blot: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Dot blot of double stranded DNA using RM231. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.
Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Flow Cytometry analysis of 5-MethylCytosine expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61470 (Blue) or with a negative control antibody
Applications for 5-MethylCytosine Antibody (RM231)
Application
Recommended Usage
Dot Blot
0.5 ug/ml - 2 ug/ml
ELISA
0.1 ug/ml - 1 ug/ml
Flow Cytometry
0.5 ug/ml-2 ug/ml
Immunocytochemistry/ Immunofluorescence
0.5 ug/ml - 2 ug/ml
Methylated DNA Immunoprecipitation
0.2 ug/ml - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine Antibody (RM231)
Product Specific Notices for 5-MethylCytosine Antibody (RM231)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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