5-MethylCytosine Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-744
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Rat, All Species
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Concentration
3.0 mg/ml
Product Specifications
Immunogen
5-Methyl cytosine conjugated to KLH.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 20808872).
Specificity
This is specific for 5-Methyl Cytosine. It was tested using immunoprecipitation against 5-Methyl Cytosine and bromodeoxyuridine (BrdU) or a control (no antigen).
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for 5-MethylCytosine Antibody
![Western Blot: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0006.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744]")
Western Blot: 5-MethylCytosine Antibody [NB100-744]
Western Blot: 5-MethylCytosine Antibody [NB100-744] - Global epigenetic changes in HFFs 7 days after hESC extract treatment. (B) Immunoblotting analysis of 5-methyl cytosine. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/Cytosine-5-Methyl-Antibody-Immunocytochemistry-Immunofluorescence-NB100-744-img0004.jpg "Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]")
Immunocytochemistry/ Immunofluorescence: 5-MethylCytosine Antibody [NB100-744]
Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody [NB100-744] - Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4 degrees C overnight using NB100-744 at a dilution of 1:200.![Western Blot: 5-MethylCytosine Antibody [NB100-744]](https://resources.bio-techne.com/images/products/5-MethylCytosine-Antibody-Western-Blot-NB100-744-img0005.jpg "Western Blot: 5-MethylCytosine Antibody [NB100-744]")
Western Blot: 5-MethylCytosine Antibody [NB100-744]
Western Blot: 5-MethylCytosine Antibody [NB100-744] - Immunoblotting analysis shows decrease in 5-methyl cytosine and increase in H3K9 acetylation levels after combined treatment. Lanes 1 to 5 were loaded with proteins (2 ug) from HFFs, hESCs, inhibitor treated HFFs and combined treated HFFs. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0012297), licensed under a CC-BY license.Applications for 5-MethylCytosine Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
25-100 ug/ml
Application Notes
Western blot images and application from PMID 20808872.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.5)
Preservative
No Preservative
Concentration
3.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine Antibody
Product Specific Notices for 5-MethylCytosine Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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