5-MethylCytosine Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42813
Key Product Details
Species Reactivity
Non-species specific
Applications
Validated:
Dot Blot, Electron Microscopy, Immunoprecipitation, Methylated DNA Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to 5-MethylCytosine / 5-mC
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 5-MethylCytosine Antibody
Dot Blot: 5-MethylCytosine Antibody [NBP2-42813] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:4000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.
![Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813]](https://resources.bio-techne.com/images/products/5-MethylCytosine-Antibody-Immunoprecipitation-NBP2-42813-img0005.jpg "Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813]")
Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813]
Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody (NBP2-42813) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.Applications for 5-MethylCytosine Antibody
Application
Recommended Usage
Dot Blot
1:100 - 1:2000
Electron Microscopy
1:10 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Specific Notices for 5-MethylCytosine Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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