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5-MethylCytosine Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42813

Novus Biologicals, part of Bio-Techne
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NBP2-42813

Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Dot Blot, Electron Microscopy, Immunoprecipitation, Methylated DNA Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to 5-MethylCytosine / 5-mC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-MethylCytosine Antibody

Dot Blot: 5-MethylCytosine Antibody [NBP2-42813] - Analysis of anti-5-mC antibody with the synthetic DNA controls . DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody at a dilution of 1:4000. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine.
Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813]

Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813]

Immunoprecipitation: 5-MethylCytosine Antibody [NBP2-42813] - Human genomic DNA (150 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody (NBP2-42813) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Applications for 5-MethylCytosine Antibody

Application
Recommended Usage

Dot Blot

1:100 - 1:2000

Electron Microscopy

1:10 - 1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 5-MethylCytosine

Alternate Names

5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)

Additional 5-MethylCytosine Products

Product Documents for 5-MethylCytosine Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-MethylCytosine Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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