53BP1 Antibody (BL-250-1H11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14667

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14667
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BL-250-1H11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 53BP1 Antibodies »

Product Summary for 53BP1 Antibody (BL-250-1H11)

Immunogen

residues 19-247 (mature protein)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for 53BP1 Antibody (BL-250-1H11)

Western Blot: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Western Blot: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Western Blot: 53BP1 Antibody [NBP3-14667] - Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] NBP3-14667 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 10 second
Immunohistochemistry: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Immunohistochemistry: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-53BP1 recombinant monoclonalantibody used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Immunohistochemistry: 53BP1 Antibody (BL-250-1H11) [NBP3-14667]

Immunohistochemistry: 53BP1 Antibody [NBP3-14667] - Sample: FFPE section of colon carcinoma. Antibody: Rabbitanti-53BP1 recombinant monoclonal antibody used at 1:250. Secondary:HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.
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Applications for 53BP1 Antibody (BL-250-1H11)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: 53BP1

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Long Name

p53 Binding Protein 1

Alternate Names

p202, TP53BP1

Gene Symbol

TP53BP1

Additional 53BP1 Products

Product Documents for 53BP1 Antibody (BL-250-1H11)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 53BP1 Antibody (BL-250-1H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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