A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59940

Novus Biologicals, part of Bio-Techne
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NBP2-59940
NBP2-59940-0.025mg
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Key Product Details

Species Reactivity

Bacteria

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 kappa Clone # SS QD1

Format

BSA Free

Concentration

1 mg/ml

View all A. aeolicus BPL/BioID2 Antibodies »

Product Specifications

Immunogen

GST fused to A. aeolicus BPL/BioID2

Reactivity Notes

Aquifex aeolicus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2 kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 (6) without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS QD1 antibody. The predicted molecular weight of BioID2-TorsinA delta E302/3 fusion protein is 65 kDa.
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free [NBP2-59940]

Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free [NBP2-59940]

Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Staining of HeLa cell line expressing doxycycline (dox) - inducible BioID2 tagged to TorsinA delta E302/3 with SS QD1 monoclonal antibody. Uninduced (-dox) or induced (+dox) cells were fixed in (A) cold methanol or (B) 4% PFA and stained with anti-BPL R40G/BioID2 SS QD1 antibody (in green). The nuclei were counter-stained with Hoechst.
Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Lane 1: lysate from untransfected HEK293 cells. Lane 2: lysate from HEK293 cells transfected with BioID2-PTEN. A specific signal for BioID2-PTEN is visible at ~94kDa.

Applications for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive Control: Cells overexpressing A. aeolicus BPL/BioID2 construct.

Reviewed Applications

Read 1 review rated 4 using NBP2-59940 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: A. aeolicus BPL/BioID2

Additional A. aeolicus BPL/BioID2 Products

Product Documents for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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