AARS2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98582
![Western Blot: AARS2 Antibody [NBP2-98582]](https://resources.bio-techne.com/images/products/AARS2-Antibody-Western-Blot-NBP2-98582-img0003.jpg "Western Blot: AARS2 Antibody [NBP2-98582]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for AARS2 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human AARS2 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for AARS2 Antibody
Western Blot: AARS2 Antibody [NBP2-98582]
Western Blot: AARS2 Antibody [NBP2-98582] - Anti-AARS2 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 107 kDa (We are unsure as to the identity of these extra bands).![Immunocytochemistry/ Immunofluorescence: AARS2 Antibody [NBP2-98582]](https://resources.bio-techne.com/images/products/AARS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98582-img0001.jpg "Immunocytochemistry/ Immunofluorescence: AARS2 Antibody [NBP2-98582]")
Immunocytochemistry/ Immunofluorescence: AARS2 Antibody [NBP2-98582]
Immunocytochemistry/Immunofluorescence: AARS2 Antibody [NBP2-98582] - Immunofluorescence staining of AARS2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARS2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Applications for AARS2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AARS2
Alternate Names
alanine tRNA ligase 2, mitochondrial (putative), alanine--tRNA ligase, alanyl-tRNA synthetase 2, mitochondrial (putative), alanyl-tRNA synthetase, mitochondrial, alaRS, bA444E17.1, COXPD8, KIAA1270, MTALARS, MT-ALARS, probable alanyl-tRNA synthetase, mitochondrial
Gene Symbol
AARS2
Additional AARS2 Products
Product Specific Notices for AARS2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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