Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Tsp232A

Concentration

1 mg/ml

View all AARSD1 Antibodies »

Product Specifications

Immunogen

Recombinant tsp23

Localization

Cytoplasm

Specificity

Detects approx 19kDa. Detects Tsp23, no cross-reactivity to p23.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for AARSD1 Antibody (Tsp232A)

Western Blot: AARSD1 Antibody (Tsp232A) [NBP2-12887]

Western Blot: AARSD1 Antibody (Tsp232A) [NBP2-12887]

Western Blot: AARSD1 Antibody (Tsp232A) [NBP2-12887] - analysis of Human Heat Shocked HeLa cell lysates showing detection of Tsp23 protein using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry: AARSD1 Antibody (Tsp232A) [NBP2-12887]

Immunohistochemistry: AARSD1 Antibody (Tsp232A) [NBP2-12887]

Immunohistochemistry: AARSD1 Antibody (Tsp232A) [NBP2-12887] - Immunohistochemistry analysis using Mouse Anti-Tsp23 Monoclonal Antibody, Clone TSp232A (NBP2-12887). Tissue: Heart Skeletal Muscle. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Tsp23 Monoclonal Antibody (NBP2-12887) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.

Applications for AARSD1 Antibody (Tsp232A)

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml of Tsp23 Antibody was sufficient for detection of tsp23 in 20 ug of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AARSD1

1 ug/mL was sufficient for detection of tsp23 in 20ug of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

alanyl-tRNA editing protein Aarsd1, alanyl-tRNA synthetase domain containing 1, Alanyl-tRNA synthetase domain-containing protein 1, MGC2744

Gene Symbol

AARSD1

Additional AARSD1 Products

Product Documents for AARSD1 Antibody (Tsp232A)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AARSD1 Antibody (Tsp232A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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