ABCA12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93466
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human, Mouse
Applications
Validated:
Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Peptide ELISA
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KDQKSYETADTSSQ corresponding to internal region (near C-Terminus) according to NP_775099.2, NP_056472.2.
Epitope
C-KDQKSYETADTSSQ
Reactivity Notes
Human and Mouse reactivity reported in scientific literature (PMID: 23931754).
Specificity
This antibody is expected to recognize both reported isoforms (NP_775099.2; NP_056472.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for ABCA12 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Peptide ELISA
Detection limit 1:1000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human placenta, skin and testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193 kDa band according to NP_775099.2 and of 257 kDa band according to NP_056472.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IP and ICC/IF usage are reported in scientific literature (PMID: 23931754). Use in immunoblotting reported in scientific literature (PMID: 23931754).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ABCA12
Alternate Names
ABC12, ATP-binding cassette 12, ATP-binding cassette transporter 12, ATP-binding cassette, sub-family A (ABC1), member 12, DKFZP434G232, EC 3.6.3, EC 3.6.3.25, FLJ41584, ichthyosis congenita II, lamellar ichthyosis B, ICR2B, LI2ATP-binding cassette sub-family A member 12
Entrez Gene IDs
26154 (Human)
Gene Symbol
ABCA12
UniProt
Additional ABCA12 Products
Product Documents for ABCA12 Antibody
Product Specific Notices for ABCA12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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