ABCG8 Antibody (1B10A5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80560

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1B10A5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ABCG8 Antibodies »

Product Summary for ABCG8 Antibody (1B10A5) - Azide and BSA Free

Immunogen

Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]

Localization

Membrane; Multi-pass membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for ABCG8 Antibody (1B10A5) - Azide and BSA Free

Western Blot: ABCG8 Antibody (1B10A5)Azide and BSA Free [NBP2-80560]

Western Blot: ABCG8 Antibody (1B10A5)Azide and BSA Free [NBP2-80560]

Western Blot: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560]

Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560]

Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560]

Immunohistochemistry: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560]

Immunohistochemistry: ABCG8 Antibody (1B10A5) - Azide and BSA Free [NBP2-80560] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706. Image from the standard format of this antibody.

Applications for ABCG8 Antibody (1B10A5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

25ug

Western Blot

1:1000
Application Notes
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCG8

Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.

Alternate Names

ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2), GBD4ATP-binding cassette, subfamily G, member 8, MGC142217, sterolin 2, sterolin-2, STSL

Gene Symbol

ABCG8

Additional ABCG8 Products

Product Documents for ABCG8 Antibody (1B10A5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ABCG8 Antibody (1B10A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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