ABCG8 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92641
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ABCG8 Antibody - BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1). MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ABCG8 Antibody - BSA Free
![Western Blot: ABCG8 AntibodyBSA Free [NBP2-92641]](https://resources.bio-techne.com/images/products/ABCG8-Antibody-Western-Blot-NBP2-92641-img0002.jpg "Western Blot: ABCG8 AntibodyBSA Free [NBP2-92641]")
Western Blot: ABCG8 AntibodyBSA Free [NBP2-92641]
Western Blot: ABCG8 Antibody [NBP2-92641] - Analysis of extracts of LO2 cells, using ABCG8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 18s.![Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody - BSA Free [NBP2-92641]](https://resources.bio-techne.com/images/products/ABCG8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92641-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody - BSA Free [NBP2-92641]")
Immunocytochemistry/ Immunofluorescence: ABCG8 Antibody - BSA Free [NBP2-92641]
Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NBP2-92641] - Analysis of HeLa cells using ABCG8 . Blue: DAPI for nuclear staining.Applications for ABCG8 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ABCG8
Transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. Specifically, ABCG8 is responsible for the selective transport of dietary cholesterol in/out of the enterocytes and in the selective liver sterol excretion into bile. ABCG8 also cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols.
ABCG8 is highly expressed in the liver and intestine. Mutated forms of ABCG8 lead to sterol accumulation and can cause atherosclerosis or sitosterolemia, a rare autosomal recessive disorder characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.
ABCG8 antibodies are useful tools for cholesterol absorbtion/sectretion studies and are fundamental to the understanding of certian liver functions.
Alternate Names
ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2), GBD4ATP-binding cassette, subfamily G, member 8, MGC142217, sterolin 2, sterolin-2, STSL
Gene Symbol
ABCG8
Additional ABCG8 Products
Product Documents for ABCG8 Antibody - BSA Free
Product Specific Notices for ABCG8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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