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ABHD14B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84472

Novus Biologicals, part of Bio-Techne
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NBP1-84472
NBP1-84472-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABHD14B Antibody

Western Blot: ABHD14B Antibody [NBP1-84472]

Western Blot: ABHD14B Antibody [NBP1-84472]

Western Blot: ABHD14B Antibody [NBP1-84472] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: ABHD14B Antibody [NBP1-84472]

Immunocytochemistry/ Immunofluorescence: ABHD14B Antibody [NBP1-84472]

Immunocytochemistry/Immunofluorescence: ABHD14B Antibody [NBP1-84472] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Immunohistochemistry-Paraffin: ABHD14B Antibody [NBP1-84472]

Immunohistochemistry-Paraffin: ABHD14B Antibody [NBP1-84472]

Immunohistochemistry-Paraffin: ABHD14B Antibody [NBP1-84472] - Staining of human pancreas shows strong cytoplasmic and nuclear positivity in exocrine glandular cells and islet cells.

Applications for ABHD14B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABHD14B

ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription

Alternate Names

abhydrolase domain containing 14B, abhydrolase domain-containing protein 14B, CCG1-interacting factor B, CIBcell cycle gene 1-interacting factor B, EC 3.-, EC 3.1.11.6, EC 3.1.21.4, MGC15429

Entrez Gene IDs

84836 (Human)

Gene Symbol

ABHD14B

UniProt

Additional ABHD14B Products

Product Documents for ABHD14B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ABHD14B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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