ABHD14B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84472
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ABHD14B Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ABHD14B Antibody
Western Blot: ABHD14B Antibody [NBP1-84472]
Western Blot: ABHD14B Antibody [NBP1-84472] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissueImmunocytochemistry/ Immunofluorescence: ABHD14B Antibody [NBP1-84472]
Immunocytochemistry/Immunofluorescence: ABHD14B Antibody [NBP1-84472] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.Immunohistochemistry-Paraffin: ABHD14B Antibody [NBP1-84472]
Immunohistochemistry-Paraffin: ABHD14B Antibody [NBP1-84472] - Staining of human pancreas shows strong cytoplasmic and nuclear positivity in exocrine glandular cells and islet cells.Applications for ABHD14B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ABHD14B
Alternate Names
abhydrolase domain containing 14B, abhydrolase domain-containing protein 14B, CCG1-interacting factor B, CIBcell cycle gene 1-interacting factor B, EC 3.-, EC 3.1.11.6, EC 3.1.21.4, MGC15429
Entrez Gene IDs
84836 (Human)
Gene Symbol
ABHD14B
UniProt
Additional ABHD14B Products
Product Documents for ABHD14B Antibody
Product Specific Notices for ABHD14B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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