ABHD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83711
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ABHD4 Antibody
Western Blot: ABHD4 Antibody [NBP1-83711]
Western Blot: ABHD4 Antibody [NBP1-83711] - Analysis in control (vector only transfected HEK293T lysate) and ABHD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: ABHD4 Antibody [NBP1-83711]
Immunocytochemistry/Immunofluorescence: ABHD4 Antibody [NBP1-83711] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-83711]
Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-83711] - Staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.Applications for ABHD4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ABHD4
Alternate Names
ABH4, abhydrolase domain containing 4, abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, EC 3.1.1.-, FLJ12816, Lyso-N-acylphosphatidylethanolamine lipase
Gene Symbol
ABHD4
Additional ABHD4 Products
Product Documents for ABHD4 Antibody
Product Specific Notices for ABHD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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