ABI1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-59845
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human abl-interactor 1 using the numbering given in entry NP_005461.2 (GeneID 10006).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ABI1 Antibody
![Western Blot: ABI1 Antibody [NB100-59845]](https://resources.bio-techne.com/images/products/ABI1-Antibody-Immunoprecipitation-NB100-59845-img0005.jpg "Western Blot: ABI1 Antibody [NB100-59845]")
Western Blot: ABI1 Antibody [NB100-59845]
Western Blot: ABI1 Antibody [NB100-59845] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ABI1 antibody NB100-59845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABI1 was efficiently immunoprecipitated by rabbit anti-ABI1 antibodies NB100-59843 and NB100-59844, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).![Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845]](https://resources.bio-techne.com/images/products/ABI1-Antibody-Immunohistochemistry-NB100-59845-img0004.jpg "Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845]")
Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845]
Immunohistochemistry-Paraffin: ABI1 Antibody [NB100-59845] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABI1 used at a dilution of 1:200 ( 1ug/ml). Detection: DABApplications for ABI1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IP not recommended. Use rabbit anti-ABI1 antibody NB100-59843 or NB100-59844.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ABI1
Long Name
Abl Interactor 1
Alternate Names
ABI-1, ABLBP4, E3B1, NAP1BP, SSH3BP, SSH3BP1
Entrez Gene IDs
10006 (Human)
Gene Symbol
ABI1
UniProt
Additional ABI1 Products
Product Documents for ABI1 Antibody
Product Specific Notices for ABI1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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