ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47870
![Immunohistochemistry-Paraffin: ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free [NBP2-47870]](https://resources.bio-techne.com/images/products/ABO-Blood-Group-A-Antigen-Antibody-HEB-20-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47870-img0001.jpg "Immunohistochemistry-Paraffin: ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free [NBP2-47870]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Agglutination, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HEB-20
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
Immunogen
Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B
Reactivity Notes
Others not known.
Localization
Cell Surface
Specificity
The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The monoclonal antibody HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This monoclonal antibody is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44632).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
Immunohistochemistry-Paraffin: ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free [NBP2-47870]
Immunohistochemistry-Paraffin: ABO, Blood Group A Antigen Antibody (HEB-20) - Azide and BSA Free [NBP2-47870] - Human Colon Carcinoma stained with Blood Group B Monoclonal Antibody (HEB-20).Applications for ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ABO, Blood Group B Antigen
Alternate Names
A transferase, A3GALNT, A3GALT1, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase;transferase B, alpha 1-3-galactosyltransferase), ABO glycosyltransferase, B transferase, B(A) alpha-1,3-galactosyltransferase, EC 2.4.1.37, EC 2.4.1.40, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, GTB, Histo-blood group A transferase, histo-blood group A2 transferase, histo-blood group ABO system transferase, Histo-blood group B transferase, NAGAT
Gene Symbol
ABO
Additional ABO, Blood Group B Antigen Products
Product Documents for ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
Product Specific Notices for ABO, Blood Group B Antigen Antibody (HEB-20) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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