Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ABT1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Activator of Basal Transcription 1 using the numbering given in entry NP_037507.1 (GeneID 29777).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABT1 Antibody

Western Blot: ABT1 Antibody [NBP2-22333]

Western Blot: ABT1 Antibody [NBP2-22333]

Western Blot: ABT1 Antibody [NBP2-22333] - Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibody NBP2-22333 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ABT1 Antibody [NBP2-22333]

Immunoprecipitation: ABT1 Antibody [NBP2-22333]

Immunoprecipitation: ABT1 Antibody [NBP2-22333] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22333 used for IP at 6 ug/mg lysate. ABT1 was also immunoprecipitated by rabbit anti-ABT1 antibody NBP2-22332. For blotting immunoprecipitated ABT1, NBP2-22333 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for ABT1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ABT1

Basal transcription of genes by RNA polymerase II requires the interaction of TATA-binding protein (TBP) with the core region of class II promoters. Studies in mouse suggest that the protein encoded by this gene likely activates basal transcription from class II promoters by interaction with TBP and the class II promoter DNA.

Alternate Names

activator of basal transcription 1, Basal transcriptional activator, hABT1, TATA-binding protein-binding protein

Gene Symbol

ABT1

UniProt

NP_037507.1 (Human)

Additional ABT1 Products

Product Documents for ABT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ABT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.