ACAA2 Antibody (12F4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26536
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12F4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human ACAA2 [UniProt P42765]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ACAA2 Antibody (12F4)
Immunohistochemistry: ACAA2 Antibody (12F4) [NBP3-26536] -
Immunohistochemistry: ACAA2 Antibody (12F4) [NBP3-26536] - Image of ACAA2 Antibody (12F4) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: ACAA2 Antibody (12F4) [NBP3-26536] -
Western Blot: ACAA2 Antibody (12F4) [NBP3-26536] - Positive Western Blot detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Mouse thymus.All lanes: ACAA2 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536] -
Immunocytochemistry/Immunofluorescence: ACAA2 Antibody (12F4) [NBP3-26536] - Staining of Hela cell with ACAA2 Antibody (12F4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 570-conjugated Goat Anti-Rabbit IgG (H+L).Applications for ACAA2 Antibody (12F4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ACAA2
Alternate Names
3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 2, acetyl-Coenzyme A acyltransferase 2, beta ketothiolase, Beta-ketothiolase, DSAEC, EC 2.3.1, EC 2.3.1.16, FLJ35992, FLJ95265, Mitochondrial 3-oxoacyl-CoA thiolase, mitochondrial 3-oxoacyl-Coenzyme A thiolase, T1
Gene Symbol
ACAA2
Additional ACAA2 Products
Product Documents for ACAA2 Antibody (12F4)
Product Specific Notices for ACAA2 Antibody (12F4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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