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ACAA2 Antibody (OTI1C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46477

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1C10

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human ACAA2(NP_006102) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACAA2 Antibody (OTI1C10)

Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477]

Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.

Applications for ACAA2 Antibody (OTI1C10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACAA2

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

Alternate Names

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 2, acetyl-Coenzyme A acyltransferase 2, beta ketothiolase, Beta-ketothiolase, DSAEC, EC 2.3.1, EC 2.3.1.16, FLJ35992, FLJ95265, Mitochondrial 3-oxoacyl-CoA thiolase, mitochondrial 3-oxoacyl-Coenzyme A thiolase, T1

Gene Symbol

ACAA2

Additional ACAA2 Products

Product Documents for ACAA2 Antibody (OTI1C10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACAA2 Antibody (OTI1C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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