ACAA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84974

Novus Biologicals, part of Bio-Techne
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NBP1-84974
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACAA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLRGVFVVAAKRTPFGAYGGLLKDFTATDLSEFAAKAALSAGKVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCGSGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACAA2 Antibody

Western Blot: ACAA2 Antibody [NBP1-84974]

Western Blot: ACAA2 Antibody [NBP1-84974]

Western Blot: ACAA2 Antibody [NBP1-84974] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: ACAA2 Antibody [NBP1-84974]

Immunocytochemistry/ Immunofluorescence: ACAA2 Antibody [NBP1-84974]

Immunocytochemistry/Immunofluorescence: ACAA2 Antibody [NBP1-84974] - Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.
Immunohistochemistry-Paraffin: ACAA2 Antibody [NBP1-84974]

Immunohistochemistry-Paraffin: ACAA2 Antibody [NBP1-84974]

Immunohistochemistry-Paraffin: ACAA2 Antibody [NBP1-84974] - Staining in human kidney and pancreas tissues using anti-ACAA2 antibody. Corresponding ACAA2 RNA-seq data are presented for the same tissues.
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Applications for ACAA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAA2

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

Alternate Names

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 2, acetyl-Coenzyme A acyltransferase 2, beta ketothiolase, Beta-ketothiolase, DSAEC, EC 2.3.1, EC 2.3.1.16, FLJ35992, FLJ95265, Mitochondrial 3-oxoacyl-CoA thiolase, mitochondrial 3-oxoacyl-Coenzyme A thiolase, T1

Entrez Gene IDs

10449 (Human)

Gene Symbol

ACAA2

UniProt

P42765 (Human)

Additional ACAA2 Products

Product Documents for ACAA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACAA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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