ACAD9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82749

Novus Biologicals, part of Bio-Techne
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NBP1-82749
NBP1-82749-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACAD9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVVHPSLADSANKFEENTYCFGRTVETLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACAD9 Antibody

Western Blot: ACAD9 Antibody [NBP1-82749]

Western Blot: ACAD9 Antibody [NBP1-82749]

Western Blot: ACAD9 Antibody [NBP1-82749] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: ACAD9 Antibody [NBP1-82749]

Western Blot: ACAD9 Antibody [NBP1-82749]

Western Blot: ACAD9 Antibody [NBP1-82749] - Analysis using Anti-ACAD9 antibody NBP1-82749 (A) shows similar pattern to independent antibody NBP2-56718 (B).
Immunocytochemistry/ Immunofluorescence: ACAD9 Antibody [NBP1-82749]

Immunocytochemistry/ Immunofluorescence: ACAD9 Antibody [NBP1-82749]

Immunocytochemistry/Immunofluorescence: ACAD9 Antibody [NBP1-82749] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
View 1 more images

Applications for ACAD9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAD9

Mitochondrial fatty acid beta-oxidation is one of the main energy-producing metabolic pathways in eukaryotes. Acyl-CoA dehydrogenases (ACADs; EC 1.3.99.13) are mitochondrial enzymes that catalyze the initial rate-limiting step in the beta-oxidation of fatty acyl-CoA. ACAD9 belongs to a group of ACADs that act on fatty acids containing 14 to 20 carbons (Zhang et al., 2002 [PubMed 12359260]).[supplied by OMIM]

Alternate Names

ACAD-9, acyl-CoA dehydrogenase family member 9, mitochondrial, acyl-CoA dehydrogenase family, member 9, acyl-Coenzyme A dehydrogenase family, member 9, EC 1.3.99, EC 1.3.99.-, EC 1.3.99.10, FLJ23533, MGC14452, NPD002

Entrez Gene IDs

28976 (Human)

Gene Symbol

ACAD9

UniProt

Q9H845 (Human)

Additional ACAD9 Products

Product Documents for ACAD9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACAD9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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