ACADM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89292

Novus Biologicals, part of Bio-Techne
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NBP1-89292
NBP1-89292-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACADM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACADM Antibody

Western Blot: ACADM Antibody [NBP1-89292]

Western Blot: ACADM Antibody [NBP1-89292]

Western Blot: ACADM Antibody [NBP1-89292] - Staining of human kidney shows high expression.
Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP1-89292]

Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP1-89292]

Immunocytochemistry/Immunofluorescence: ACADM Antibody [NBP1-89292] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human kidney, liver, lymph node and skin using Anti-ACADM antibody NBP1-89292 (A) shows similar protein distribution across tissues to independent antibody NBP1-89293 (B).
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Applications for ACADM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACADM

ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Alternate Names

ACAD1, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, EC 1.3.99, EC 1.3.99.3, FLJ18227, FLJ99884, MCADFLJ93013, MCADH, medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene Symbol

ACADM

Additional ACADM Products

Product Documents for ACADM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACADM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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