ACADSB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48739

Novus Biologicals, part of Bio-Techne
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NBP2-48739
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ACADSB Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACADSB Antibody

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-48739]

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-48739]

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-48739] - Staining in human liver and placenta tissues using anti-ACADSB antibody. Corresponding ACADSB RNA-seq data are presented for the same tissues.
Western Blot: ACADSB Antibody [NBP2-48739]

Western Blot: ACADSB Antibody [NBP2-48739]

Western Blot: ACADSB Antibody [NBP2-48739] - Analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: ACADSB Antibody [NBP2-48739]

Immunocytochemistry/ Immunofluorescence: ACADSB Antibody [NBP2-48739]

Immunocytochemistry/Immunofluorescence: ACADSB Antibody [NBP2-48739] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.
View 3 more images

Applications for ACADSB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACADSB

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes thatcatalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids.Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB geneproduct has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, butalso reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. ThecDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield amature peptide of approximately 43.7-KDa. (provided by RefSeq)

Long Name

Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

Alternate Names

2-MEBCAD, EC 1.3.8.5, SBCAD

Gene Symbol

ACADSB

Additional ACADSB Products

Product Documents for ACADSB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACADSB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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