Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 55D5

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Recombinant human ACAT2 (1-397aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ACAT2 Antibody (55D5) - BSA Free

Western Blot: ACAT2 Antibody (55D5)BSA Free [NBP2-42614]

Western Blot: ACAT2 Antibody (55D5)BSA Free [NBP2-42614]

Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein
Immunocytochemistry/ Immunofluorescence: ACAT2 Antibody (55D5) - BSA Free [NBP2-42614]

Immunocytochemistry/ Immunofluorescence: ACAT2 Antibody (55D5) - BSA Free [NBP2-42614]

Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (55D5) [NBP2-42614] - ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: ACAT2 Transfected 293T cell lysate
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Applications for ACAT2 Antibody (55D5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT2

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Gene Symbol

ACAT2

UniProt

NP_005882 (Human)

Additional ACAT2 Products

Product Documents for ACAT2 Antibody (55D5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ACAT2 Antibody (55D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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