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ACAT2 Antibody (OTI3E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02588

Novus Biologicals, part of Bio-Techne
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NBP2-02588
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E2

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACAT2 Antibody (OTI3E2)

Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ACAT2 Antibody (OTI3E2) [NBP2-02588]

Immunocytochemistry/ Immunofluorescence: ACAT2 Antibody (OTI3E2) [NBP2-02588]

Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.
Immunohistochemistry-Paraffin: ACAT2 Antibody (OTI3E2) [NBP2-02588]

Immunohistochemistry-Paraffin: ACAT2 Antibody (OTI3E2) [NBP2-02588]

Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.

Applications for ACAT2 Antibody (OTI3E2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACAT2

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Entrez Gene IDs

39 (Human)

Gene Symbol

ACAT2

Additional ACAT2 Products

Product Documents for ACAT2 Antibody (OTI3E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACAT2 Antibody (OTI3E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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