Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54)

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE55-54) [NBP3-33134] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33134) at 1/1,000 dilution.

Lane 1: SH-SY5Y whole cell lysate
Lane 2: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes whole cell lysate
Lane 3: SH-SY5Y treated with pp for 1 hour whole cell lysate
Lane 4: SH-SY5Y treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 277 kDa
Observed band size: 277 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33134) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.