Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95)

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135]

Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (JE63-95) [NBP3-33135] - Western blot analysis of Acetyl-CoA Carboxylase alpha/ACACA on different lysates with Rabbit anti-Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-33135) at 1/2,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 treated with 0.5uM Oligomycin for 30 minutes then treated with pp for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 265 kDa
Observed band size: 265 kDa

Exposure time: 1 minutes 14 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33135) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.