Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33573

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 3P2E4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Acetyl-CoA Carboxylase Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S79 of human Acetyl-CoA Carboxylase

Modification

p Ser79

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

265 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Western Blot: Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4) [NBP3-33573] -

Western Blot: Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4) [NBP3-33573] - Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-CoA Carboxylase Rabbit mAb at 1:7000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:2000 - 1:8000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-CoA Carboxylase

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4).

Alternate Names

ACAC, Acac1, ACACAD, ACC, ACC1, ACCA, ACC-alpha, ACCalphaACACalpha, acetyl-CoA carboxylase 1, acetyl-Coenzyme A carboxylase alpha, hACC1

Gene Symbol

ACACA

Additional Acetyl-CoA Carboxylase Products

Product Documents for Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetyl-CoA Carboxylase [p Ser79] Antibody (3P2E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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