Acetyl CoA synthetase Antibody (2A10)
Novus Biologicals, part of Bio-Techne | Catalog # H00055902-M09
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2A10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ACSS2 (NP_061147.1, 32 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPEVSRSAHVPSLQRYRELHRRSVEEPREFWGDIAKEFYWKTPCPGPFLRYNFDVTKGKIFIEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEP
Specificity
Reacts with acyl-CoA synthetase short-chain family member 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Acetyl CoA synthetase Antibody (2A10)
![Western Blot: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-2A10-Western-Blot-H00055902-M09-img0003.jpg "Western Blot: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]")
Western Blot: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]
Western Blot: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09] - Analysis of ACSS2 expression in transfected 293T cell line by ACSS2 monoclonal antibody (M09), clone 2A10. Lane 1: ACSS2 transfected lysate (Predicted MW: 67.6 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-2A10-Immunocytochemistry-Immunofluorescence-H00055902-M09-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]")
Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09]
Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09] - Analysis of monoclonal antibody to ACSS2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Acetyl CoA synthetase Antibody (2A10) [H00055902-M09] - Detection limit for recombinant GST tagged ACSS2 is 1 ng/ml as a capture antibody.
Applications for Acetyl CoA synthetase Antibody (2A10)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Acetyl CoA synthetase
Alternate Names
ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1
Entrez Gene IDs
55902 (Human)
Gene Symbol
ACSS2
UniProt
Additional Acetyl CoA synthetase Products
Product Documents for Acetyl CoA synthetase Antibody (2A10)
Product Specific Notices for Acetyl CoA synthetase Antibody (2A10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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