Acetyl CoA synthetase Antibody (4N5V4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15447
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4N5V4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Acetyl CoA synthetase Antibody (4N5V4)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Acetyl CoA synthetase (Q9NR19). LHRRSVEEPREFWGDIAKEFYWKTPCPGPFLRYNFDVTKGKIFIEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTQITYHQLLVQVCQFSN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Acetyl CoA synthetase Antibody (4N5V4)
![Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-4N5V4-Western-Blot-NBP3-15447-img0001.jpg "Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]")
Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]
Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447] - Western blot analysis of extracts of various cell lines, using Acetyl CoA synthetase Rabbit mAb (NBP3-15447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]](https://resources.bio-techne.com/images/products/Acetyl-CoA-synthetase-Antibody-4N5V4-Western-Blot-NBP3-15447-img0002.jpg "Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]")
Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447]
Western Blot: Acetyl CoA synthetase Antibody (4N5V4) [NBP3-15447] - Western blot analysis of extracts of HepG2 cells, using Acetyl CoA synthetase Rabbit mAb (NBP3-15447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for Acetyl CoA synthetase Antibody (4N5V4)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Acetyl CoA synthetase
Alternate Names
ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1
Gene Symbol
ACSS2
Additional Acetyl CoA synthetase Products
Product Documents for Acetyl CoA synthetase Antibody (4N5V4)
Product Specific Notices for Acetyl CoA synthetase Antibody (4N5V4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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