Acetyl CoA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90276

Novus Biologicals, part of Bio-Techne
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NBP1-90276
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Acetyl CoA synthetase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PGETTQITYHQLLVQVCQFSNVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCSLLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQ

Reactivity Notes

Reactivity reported in scientific literature (PMID: 20232292), Mouse reactivity reported in the literature (PMID: 25584894)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Acetyl CoA synthetase Antibody

Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276]

Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276]

Western Blot: Acetyl CoA synthetase Antibody [NBP1-90276] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276]

Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276]

Immunocytochemistry/Immunofluorescence: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276]

Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276]

Immunohistochemistry-Paraffin: Acetyl CoA synthetase Antibody [NBP1-90276] - Staining of human tonsil shows no positivity in lymphocytes as expected.
View 3 more images

Applications for Acetyl CoA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acetyl CoA synthetase

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1

Entrez Gene IDs

55902 (Human)

Gene Symbol

ACSS2

UniProt

Q9NR19 (Human)

Additional Acetyl CoA synthetase Products

Product Documents for Acetyl CoA synthetase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Acetyl CoA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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