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Acetyl CoA synthetase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15466

Novus Biologicals, part of Bio-Techne
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NBP3-15466-100ul
NBP3-15466-20ul

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Acetyl CoA synthetase Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human Acetyl CoA synthetase (NP_061147.1). VDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acetyl CoA synthetase Antibody - BSA Free

Western Blot: Acetyl CoA synthetase AntibodyBSA Free [NBP3-15466]

Western Blot: Acetyl CoA synthetase AntibodyBSA Free [NBP3-15466]

Western Blot: Acetyl CoA synthetase Antibody [NBP3-15466] - Western blot analysis of extracts of various cell lines, using Acetyl CoA synthetase antibody (NBP3-15466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for Acetyl CoA synthetase Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl CoA synthetase

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

ACAS2, AceCS, acetate thiokinase, acetate-CoA ligase, Acetate--CoA ligase, Acetyl-CoA synthetase, acetyl-Coenzyme A synthetase 2 (ADP forming), acetyl-coenzyme A synthetase, cytoplasmic, ACSA, ACSACECS, Acyl-activating enzyme, acyl-CoA synthetase short-chain family member 2EC 6.2.1.1, cytoplasmic acetyl-coenzyme A synthetase, dJ1161H23.1, DKFZp762G026, EC 6.2.1

Gene Symbol

ACSS2

Additional Acetyl CoA synthetase Products

Product Documents for Acetyl CoA synthetase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetyl CoA synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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